From: Subject: Karyotyping Activity Date: Thu, 28 Feb 2008 23:41:07 -0500 MIME-Version: 1.0 Content-Type: multipart/related; type="text/html"; boundary="----=_NextPart_000_0046_01C87A63.63BD23F0" X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 This is a multi-part message in MIME format. ------=_NextPart_000_0046_01C87A63.63BD23F0 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Content-Location: http://www.biology.arizona.edu/human_bio/activities/karyotyping/karyotyping.html Karyotyping Activity
=20

Karyotyping = Activity=20

Introduction=20

This exercise is a simulation of human karyotyping using = digital=20 images of chromosomes from actual human genetic studies. You = will be=20 arranging chromosomes into a completed karyotype, and = interpreting=20 your findings just as if you were working in a genetic = analysis=20 program at a hospital or clinic. Karyotype analyses are = performed=20 over 400,000 times per year in the U.S. and Canada. Imagine = that you=20 were performing these analyses for real people, and that = your=20 conclusions would drastically affect their lives.=20

G Banding =

During mitosis, the 23 pairs of human chromosomes = condense and=20 are visible with a light microscope. A karyotype analysis = usually=20 involves blocking cells in mitosis and staining the = condensed=20 chromosomes with Giemsa dye. The dye stains regions of = chromosomes=20 that are rich in the base pairs Adenine (A) and Thymine (T)=20 producing a dark band. A common misconception is that bands=20 represent single genes, but in fact the thinnest bands = contain over=20 a million base pairs and potentially hundreds of genes. For = example,=20 the size of one small band is about equal to the entire = genetic=20 information for one bacterium. =

=20 =20

The analysis involves comparing chromosomes for their = length, the=20 placement of centromeres (areas where the two chromatids are = joined), and the location and sizes of G-bands. You will=20 electronically complete the karyotype for three individuals = and look=20 for abnormalities that could explain the phenotype.=20

Your = assignment=20

This exercise is designed as an introduction to genetic = studies=20 on humans. Karyotyping is one of many techniques that allow = us to=20 look for several thousand possible genetic diseases in = humans.

You will evaluate 3 patients' case histories, complete = their=20 karyotypes, and diagnose any missing or extra chromosomes. = Then=20 you'll conduct research on the internet to find web sites = that cover=20 some aspect of human genetics. If this is an assignment for = a class,=20 you should turn in a total of 7 answers on paper (2 for each = patient, 1 for the internet search). =

The=20 Biology Project
The University of Arizona
Tuesday, = June 9,=20 1998
Contact the=20 Development Team

http://www.biology.arizona.edu
All contents = copyright=20 =A9 1996. All rights reserved.

CREDITS=20

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